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Article

Introducing the Stem Cell ASCL2 Reporter STAR into Intestinal Organoids

8 Oct 2020

Abstract excerpt

Patient-derived organoids maintain functional and phenotypic characteristics of the original tissue such as cell-type diversity. Here, we provide protocols on how to label intestinal (cancer) stem cells by integrating the stem cell ASCL2 reporter (STAR) into human and mouse genomes via two different strategies: (1) lentiviral transduction or (2) transposon-based integration. Organoid technology, in combination...

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PMID: 33377020
DOI: 10.1016/j.xpro.2020.100126

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