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Article

Development of a multiplexed targeted mass spectrometry assay for LRRK2-phosphorylated Rabs and Ser910/Ser935 biomarker sites.

The Biochemical journal - 29 Jan 2021

Nirujogi Raja S, Tonelli Francesca, Taylor Matthew, Lis Pawel, Zimprich Alexander, Sammler Esther, Alessi Dario R

Abstract excerpt

Mutations that increase the protein kinase activity of LRRK2 are one of the most common causes of familial Parkinson's disease. LRRK2 phosphorylates a subset of Rab GTPases within their Switch-II motif, impacting interaction with effectors. We describe and validate a new, multiplexed targeted mass spectrometry assay to quantify endogenous levels of LRRK2-phosphorylated Rab substrates (Rab1, Rab3, Rab8, Rab10,...

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Identifiers

PMID: 33367571
DOI: 10.1042/BCJ20200930
PMCID: PMC7833208

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Development of a multiplexed targeted mass spectrometry assay for LRRK2-phosphorylated Rabs and Ser910/Ser935 biomarker sites.PMID 33367571Read ↗

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