Article
Human Peripheral Blood Neutrophil Isolation for Interrogating the Parkinson's Associated LRRK2 Kinase Pathway by Assessing Rab10 Phosphorylation.
Journal of visualized experiments : JoVE - 21 Mar 2020
Fan Ying, Tonelli Francesca, Padmanabhan Shalini, Baptista Marco A S, Riley Lindsey, Smith Danielle, Marras Connie, Howden Andrew, Alessi Dario R, Sammler Esther
Abstract excerpt
The leucine rich repeat kinase 2 (LRRK2) is the most frequently mutated gene in hereditary Parkinson' disease (PD) and all pathogenic LRRK2 mutations result in hyperactivation of its kinase function. Here, we describe an easy and robust assay to quantify LRRK2 kinase pathway activity in human peripheral blood neutrophils by measuring LRRK2-controlled phosphorylation of one of its physiological substrates, Rab10...
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