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Article

Accurate MS-based Rab10 Phosphorylation Stoichiometry Determination as Readout for LRRK2 Activity in Parkinson's Disease.

Molecular & cellular proteomics : MCP - 1 Sept 2020

Karayel Özge, Tonelli Francesca, Virreira Winter Sebastian, Geyer Phillip E, Fan Ying, Sammler Esther M, Alessi Dario R, Steger Martin, Mann Matthias

Abstract excerpt

Pathogenic mutations in the Leucine-rich repeat kinase 2 (LRRK2) are the predominant genetic cause of Parkinson's disease (PD). They increase its activity, resulting in augmented Rab10-Thr73 phosphorylation and conversely, LRRK2 inhibition decreases pRab10 levels. Currently, there is no assay to quantify pRab10 levels for drug target engagement or patient stratification. To meet this challenge, we developed an...

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Identifiers

PMID: 32601174
DOI: 10.1074/mcp.RA120.002055
PMCID: PMC8143643

Genes

VPS35

Variants

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Accurate MS-based Rab10 Phosphorylation Stoichiometry Determination as Readout for LRRK2 Activity in Parkinson's Disease.PMID 32601174Read ↗

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