Article
Rhythmic adenosine triphosphate release from the cyanobacterial circadian clock protein KaiC revealed by real-time monitoring of bioluminescence using firefly luciferase.
Genes to cells : devoted to molecular & cellular mechanisms - 1 Feb 2021
Mutoh Risa, Iwata Keita, Iida Takahiro, Ishiura Masahiro, Onai Kiyoshi
Abstract excerpt
The cyanobacterial circadian clock is composed of three clock proteins, KaiA, KaiB and KaiC. This KaiABC clock system can be reconstituted in vitro in the presence of adenosine triphosphate (ATP) and Mg2+ , and shows circadian rhythms in the phosphorylation level and ATPase activity of KaiC. Previously, we found that ATP regulates a complex formation between KaiB and KaiC, and KaiC releases ATP from KaiC itself...
Topics
- Adenosine Triphosphatases
- Adenosine Triphosphate
- Amino Acid Motifs
- Bacterial Proteins
- Biological Assay
- Circadian Clocks
- Circadian Rhythm Signaling Peptides and Proteins
- Luciferases, Firefly
- Luminescence