Article
Mutation of alanine-422 in KaiC leads to a low amplitude of rhythm in the reconstituted cyanobacterial circadian clock.
The Journal of general and applied microbiology - 17 Jun 2020
Nagata Kazuki, Oyama Katsuaki, Ota Atsushi, Azai Chihiro, Terauchi Kazuki
Abstract excerpt
The cyanobacterial circadian oscillator can be reconstituted by mixing the purified clock proteins KaiA, KaiB, and KaiC with ATP in vitro, leading to a 24-h oscillation of KaiC phosphorylation. The cyanobacterial mutant pr1 carrying valine instead of alanine at position 422 of KaiC (KaiC-A422V) l...
Topics
- Amino Acid Substitution
- Bacterial Proteins
- Circadian Clocks
- Circadian Rhythm
- Circadian Rhythm Signaling Peptides and Proteins
- Mutation
- Phosphorylation
- Recombinant Proteins
- Synechococcus