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Article

Reconstitution of circadian oscillation of cyanobacterial KaiC phosphorylation in vitro.

Science (New York, N.Y.) - 15 Apr 2005

Nakajima Masato, Imai Keiko, Ito Hiroshi, Nishiwaki Taeko, Murayama Yoriko, Iwasaki Hideo, Oyama Tokitaka, Kondo Takao

Abstract excerpt

Kai proteins globally regulate circadian gene expression of cyanobacteria. The KaiC phosphorylation cycle, which persists even without transcription or translation, is assumed to be a basic timing process of the circadian clock. We have reconstituted the self-sustainable oscillation of KaiC phosphorylation in vitro by incubating KaiC with KaiA, KaiB, and adenosine triphosphate. The period of the in vitro...

Topics

Adenosine Triphosphate
Bacterial Proteins
Circadian Rhythm
Circadian Rhythm Signaling Peptides and Proteins
Genes, Bacterial
Luminescence
Mutation
Phosphorylation
Recombinant Proteins
Synechococcus

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Identifiers

PMID: 15831759
DOI: 10.1126/science.1108451

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-15831759-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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Reconstitution of circadian oscillation of cyanobacterial KaiC phosphorylation in vitro.PMID 15831759Read ↗

KaiA-stimulated KaiC phosphorylation in circadian timing loops in cyanobacteria.Semantic proximity Read ↗

ATPase activity of KaiC determines the basic timing for circadian clock of cyanobacteria.Semantic proximity Read ↗

Transcriptional autoregulation by phosphorylated and non-phosphorylated KaiC in cyanobacterial circadian rhythms.Semantic proximity Read ↗

Circadian clocks go in vitro: purely post-translational oscillators in cyanobacteria.Semantic proximity Read ↗

Intrinsic period stability of the cyanobacterial circadian oscillator across in vitro and in vivo conditions.Semantic proximity Read ↗

Tuning the circadian period of cyanobacteria up to 6.6 days by the single amino acid substitutions in KaiC.Semantic proximity Read ↗

Intramolecular regulation of phosphorylation status of the circadian clock protein KaiC.Semantic proximity Read ↗

Intermolecular associations determine the dynamics of the circadian KaiABC oscillator.Semantic proximity Read ↗

The recovery of KaiA's activity depends on its N-terminal domain and KaiB in the cyanobacterial circadian clock.Semantic proximity Read ↗

Diversity of KaiC-based timing systems in marine Cyanobacteria.Semantic proximity Read ↗

Rhythmic adenosine triphosphate release from the cyanobacterial circadian clock protein KaiC revealed by real-time monitoring of bioluminescence using firefly luciferase.Semantic proximity Read ↗

Mutation of alanine-422 in KaiC leads to a low amplitude of rhythm in the reconstituted cyanobacterial circadian clock.Semantic proximity Read ↗

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