Article
An atypical BRCT-BRCT interaction with the XRCC1 scaffold protein compacts human DNA Ligase IIIα within a flexible DNA repair complex.
Nucleic acids research - 11 Jan 2021
Hammel Michal, Rashid Ishtiaque, Sverzhinsky Aleksandr, Pourfarjam Yasin, Tsai Miaw-Sheue, Ellenberger Tom, Pascal John M, Kim In-Kwon, Tainer John A, Tomkinson Alan E
Abstract excerpt
The XRCC1-DNA ligase IIIα complex (XL) is critical for DNA single-strand break repair, a key target for PARP inhibitors in cancer cells deficient in homologous recombination. Here, we combined biophysical approaches to gain insights into the shape and conformational flexibility of the XL as well as XRCC1 and DNA ligase IIIα (LigIIIα) alone. Structurally-guided mutational analyses based on the crystal structure of...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.