Article
Comparison of Polymerase Chain Reaction-Restriction Fragment Length Polymorphism, Immunohistochemistry, and DNA Sequencing for the Detection of IDH1 Mutations in Gliomas.
Asian Pacific journal of cancer prevention : APJCP - 1 Nov 2020
Malueka Rusdy Ghazali, Theresia Emilia, Fitria Fitria, Argo Ibnu Widya, Donurizki Aditya Dwi, Shaleh Sabillal, Innayah Meutia Rizki, Wicaksono Adiguno Suryo, Dananjoyo Kusumo, Asmedi Ahmad, Hartanto Rachmat Andi, Dwianingsih Ery Kus
Abstract excerpt
BACKGROUND: IDH1 mutation shows diagnostic, prognostic, and predictive value in gliomas. Direct Sanger sequencing is considered the gold standard to detect IDH1 mutation. However, this technology is not available in most neuropathological centers in developing countries such as Indonesia. Immunohistochemistry (IHC) and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) have also been...
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