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Article

Customized optical mapping by CRISPR-Cas9 mediated DNA labeling with multiple sgRNAs.

Nucleic acids research - 25 Jan 2021

Abid Heba Z, Young Eleanor, McCaffrey Jennifer, Raseley Kaitlin, Varapula Dharma, Wang Hung-Yi, Piazza Danielle, Mell Joshua, Xiao Ming

Abstract excerpt

Whole-genome mapping technologies have been developed as a complementary tool to provide scaffolds for genome assembly and structural variation analysis (1,2). We recently introduced a novel DNA labeling strategy based on a CRISPR-Cas9 genome editing system, which can target any 20bp sequences. The labeling strategy is specifically useful in targeting repetitive sequences, and sequences not accessible to other...

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Identifiers

PMID: 33231685
DOI: 10.1093/nar/gkaa1088
PMCID: PMC7826249

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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