Article
Application of CRISPR/Cas9 Nuclease in Amphioxus Genome Editing.
Genes - 5 Nov 2020
Su Liuru, Shi Chenggang, Huang Xin, Wang Yiquan, Li Guang
Abstract excerpt
The cephalochordate amphioxus is a promising animal model for studying the origin of vertebrates due to its key phylogenetic position among chordates. Although transcription activator-like effector nucleases (TALENs) have been adopted in amphioxus genome editing, its labor-intensive construction of TALEN proteins limits its usage in many laboratories. Here we reported an application of the CRISPR/Cas9 system, a...
Topics
- Animals
- CRISPR-Associated Protein 9
- CRISPR-Cas Systems
- Clustered Regularly Interspaced Short Palindromic Repeats
- Endonucleases
- Gene Editing
- Genome, Plant
- Genotype
- Lancelets
- Phenotype
- Phylogeny