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Article

Environmental and Physiological Factors Affecting High-Throughput Measurements of Bacterial Growth.

mBio - 20 Oct 2020

Atolia Esha, Cesar Spencer, Arjes Heidi A, Rajendram Manohary, Shi Handuo, Knapp Benjamin D, Khare Somya, Aranda-Díaz Andrés, Lenski Richard E, Huang Kerwyn Casey

Abstract excerpt

Bacterial growth under nutrient-rich and starvation conditions is intrinsically tied to the environmental history and physiological state of the population. While high-throughput technologies have enabled rapid analyses of mutant libraries, technical and biological challenges complicate data collection and interpretation. Here, we present a framework for the execution and analysis of growth measurements with...

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Identifiers

PMID: 33082255
DOI: 10.1128/mBio.01378-20
PMCID: PMC7587430

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