Article
Automated imaging with ScanLag reveals previously undetectable bacterial growth phenotypes.
Nature methods - 1 Sept 2010
Levin-Reisman Irit, Gefen Orit, Fridman Ofer, Ronin Irine, Shwa David, Sheftel Hila, Balaban Nathalie Q
Abstract excerpt
We developed an automated system, ScanLag, that measures in parallel the delay in growth (lag time) and growth rate of thousands of cells. Using ScanLag, we detected small subpopulations of bacteria with dramatically increased lag time upon starvation. By screening a library of Escherichia coli deletion mutants, we achieved two-dimensional mapping of growth characteristics, which showed that ScanLag enables...
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