Article
Deep amplicon sequencing for culture-free prediction of susceptibility or resistance to 13 anti-tuberculous drugs.
The European respiratory journal - 1 Mar 2021
Jouet Agathe, Gaudin Cyril, Badalato Nelly, Allix-Béguec Caroline, Duthoy Stéphanie, Ferré Alice, Diels Maren, Laurent Yannick, Contreras Sandy, Feuerriegel Silke, Niemann Stefan, André Emmanuel, Kaswa Michel K, Tagliani Elisa, Cabibbe Andrea, Mathys Vanessa, Cirillo Daniela, de Jong Bouke C, Rigouts Leen, Supply Philip
Abstract excerpt
Conventional molecular tests for detecting Mycobacterium tuberculosis complex (MTBC) drug resistance on clinical samples cover a limited set of mutations. Whole-genome sequencing (WGS) typically requires culture.Here, we evaluated the Deeplex Myc-TB targeted deep-sequencing assay for prediction of resistance to 13 anti-tuberculous drugs/drug classes, directly applicable on sputum.With MTBC DNA tests, the limit of...
Topics
- Antitubercular Agents
- Humans
- Microbial Sensitivity Tests
- Mutation
- Mycobacterium tuberculosis
- Pharmaceutical Preparations
- Tuberculosis
- Tuberculosis, Multidrug-Resistant