Article
Exploiting Drosophila melanogaster Wing Imaginal Disc Eversion to Screen for New EMT Effectors.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021
Golenkina Sofia, Manhire-Heath Rosemary, Murray Michael J
Abstract excerpt
In the early stages of Drosophila melanogaster (Drosophila) metamorphosis, a partial epithelial-mesenchymal transition (pEMT) takes place in the peripodial epithelium of wing imaginal discs. Blocking this pEMT results in adults with internalized wings and missing thoracic tissue. Using peripodial GAL4 drivers, GAL80ts temporal control, and UAS RNAi transgenes, one can use these phenotypes to screen for genes...
Topics
- Animals
- Drosophila Proteins
- Drosophila melanogaster
- Epithelial-Mesenchymal Transition
- Genetic Techniques
- Imaginal Discs
- Phenotype
- Tissue Culture Techniques
- Wings, Animal