Article
Quantitative genetic screening reveals a Ragulator-FLCN feedback loop that regulates the mTORC1 pathway.
Science signaling - 15 Sept 2020
De Zan Erica, van Stiphout Ruud, Gapp Bianca V, Blomen Vincent A, Brummelkamp Thijn R, Nijman Sebastian M B
Abstract excerpt
Forward genetic screens in mammalian cell lines, such as RNAi and CRISPR-Cas9 screens, have made major contributions to the elucidation of diverse signaling pathways. Here, we exploited human haploid cells as a robust comparative screening platform and report a set of quantitative forward genetic screens for identifying regulatory mechanisms of mTORC1 signaling, a key growth control pathway that senses diverse...
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