Article
Determining the Orientation of Porins in Planar Lipid Bilayers.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021
Ionescu Sandra A, Lee Sejeong, Bayley Hagan
Abstract excerpt
Single-channel planar lipid bilayer (PLB) recording of bacterial porins has revealed molecular details of transport across the outer membrane of Gram-negative bacteria, including antibiotic permeation and protein translocation. To explore directional transport processes across cellular membranes, the orientation of porins or other pore-forming proteins must be established in a lipid bilayer prior to...
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