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Article

Using Mutability Landscapes To Guide Enzyme Thermostabilization.

Chembiochem : a European journal of chemical biology - 5 Jan 2021

Guo Chao, Ni Yan, Biewenga Lieuwe, Pijning Tjaard, Thunnissen Andy-Mark W H, Poelarends Gerrit J

Abstract excerpt

Thermostabilizing enzymes while retaining their activity and enantioselectivity for applied biocatalysis is an important topic in protein engineering. Rational and computational design strategies as well as directed evolution have been used successfully to thermostabilize enzymes. Herein, we describe an alternative mutability-landscape approach that identified three single mutations (R11Y, R11I and A33D) within...

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PMID: 32790123
DOI: 10.1002/cbic.202000442
PMCID: PMC7821111

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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