Article
Dynamic single-cell phenotyping of immune cells using the microfluidic platform DropMap.
Nature protocols - 1 Sept 2020
Bounab Yacine, Eyer Klaus, Dixneuf Sophie, Rybczynska Magda, Chauvel Cécile, Mistretta Maxime, Tran Trang, Aymerich Nathan, Chenon Guilhem, Llitjos Jean-François, Venet Fabienne, Monneret Guillaume, Gillespie Iain A, Cortez Pierre, Moucadel Virginie, Pachot Alexandre, Troesch Alain, Leissner Philippe, Textoris Julien, Bibette Jérôme, Guyard Cyril, Baudry Jean, Griffiths Andrew D, Védrine Christophe
Abstract excerpt
Characterization of immune responses is currently hampered by the lack of systems enabling quantitative and dynamic phenotypic characterization of individual cells and, in particular, analysis of secreted proteins such as cytokines and antibodies. We recently developed a simple and robust microfluidic platform, DropMap, to measure simultaneously the kinetics of secretion and other cellular characteristics,...
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