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Chromatographic clarification overcomes chromatin‐mediated hitch‐hiking interactions on Protein A capture column

24 Jul 2020

Abstract excerpt

Abstract Protein A capture chromatography is a critical unit operation in the clearance of host cell protein (HCP) impurities in monoclonal antibody (mAb) purification processes. Though one of the most effective purification steps, variable levels of protein impurities are often observed in the eluate. Coelution of HCP impurities is suggested to be strongly affected by the presence of chromatin complexes (Gagnon...

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Share to Monoclonal and Polyclonal Antibodies Research
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PMID: 32706389
DOI: 10.1002/bit.27513

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