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Continuous precipitation of IgG from CHO cell culture supernatant in a tubular reactor

17 Mar 2015

Abstract excerpt

We successfully transferred a two-stage batch precipitation-based antibody capture step to continuous mode using continuous tubular reactors. The precipitation process solely employs a cheap mineral salt (CaCl2 ) and an organic solvent (ethanol) and could replace the costly protein A capture step in the purification of recombinant antibodies from cell culture supernatant. The time from startup untill attaining...

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PMID: 25781580
DOI: 10.1002/biot.201400608

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