Article
The construction of CRISPR-Cas9 system for endophytic Phomopsis liquidambaris and its PmkkA-deficient mutant revealing the effect on rice.
Fungal genetics and biology : FG & B - 1 Mar 2020
Huang Peng-Wei, Yang Qian, Zhu Ya-Li, Zhou Jun, Sun Kai, Mei Yan-Zhen, Dai Chuan-Chao
Abstract excerpt
The endophytic fungus Phomopsis liquidambaris efficiently promotes the nitrogen metabolism and growth of host plants such as rice and peanut. However, a lack of genetic tools limits further research regarding the mechanisms of interaction between P. liquidambaris and its host plants. Herein, a CRISPR/Cas9 system for targeted gene disruption in this strain was first constructed and optimized. The knock-out...
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