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Article

Engineering of a Bacillus alpha-amylase with improved thermostability and calcium independency.

Applied biochemistry and biotechnology - 1 Sept 2010

Ghollasi Marzieh, Khajeh Khosro, Naderi-Manesh Hossein, Ghasemi Atiyeh

Abstract excerpt

Successful industrial use of amylases requires that they are sufficiently stable and active at application conditions, e.g., at high temperature in starch-liquefaction process. In the present study, site-directed mutagenesis was used to enhance the thermal stability and calcium independency of a mesophilic alpha-amylase from Bacillus megaterium WHO. Mutations (A53S and H58I) were designed at the calcium-binding...

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Identifiers

PMID: 20177823
DOI: 10.1007/s12010-009-8879-2

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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