Article
Targeted gene deletion in Brettanomyces bruxellensis with an expression-free CRISPR-Cas9 system.
Applied microbiology and biotechnology - 1 Aug 2020
Varela Cristian, Bartel Caroline, Onetto Cristobal, Borneman Anthony
Abstract excerpt
The ability to genetically manipulate microorganisms has been essential for understanding their biology and metabolism. Targeted genome editing relies on highly efficient homologous recombination, and while this is readily observed in the yeast Saccharomyces cerevisiae, most non-conventional yeast species do not display this trait and remain recalcitrant to targeted editing methods. CRISPR-based editing can...
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