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CRISPR-Cas9 mediated engineering of Bacillus licheniformis for industrial production of (2R,3S)-butanediol.

Biotechnology progress - 1 Jan 2021

Song Chan Woo, Rathnasingh Chelladurai, Park Jong Myoung, Kwon Mina, Song Hyohak

Abstract excerpt

Bacillus lichenformis is an industrially promising generally recognized as safe (GRAS) strain that can be used for the production of a valuable chemical, 2,3-butanediol (BDO). Conventional gene deletion vectors and/or methods are time-consuming and have poor efficiency. Therefore, clustered regularly interspaced short palindromic repeat (CRISPR)-Cas9 mediated homologous recombination was used to engineer a newly...

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Bacterial Proteins
CRISPR-Cas Systems
Fermentation
GTP-Binding Proteins
Isocitrate Dehydrogenase

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Metabolic Engineering

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PMID: 32964665
DOI: 10.1002/btpr.3072

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