Article
Virus-Induced Gene Silencing in Lilies Using Cucumber Mosaic Virus Vectors.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2020
Tasaki Keisuke, Yamagishi Masumi, Masuta Chikara
Abstract excerpt
Virus-induced gene silencing (VIGS) systems are effective for rapid analysis of gene functions in plants that require a long period of growth such as Lilium. We successfully developed a VIGS system using the cucumber mosaic virus (HL strain, CMV-HL) vector to induce RNA silencing of the L. leichtlinii phytoene desaturase gene (LlPDS), where at 30 days postinoculation (dpi), photo-bleaching was observed in the...
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