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Enhancement of virus-induced gene silencing in tomato by low temperature and low humidity.

Molecules and cells - 28 Feb 2006

Fu Da-Qi, Zhu Ben-Zhong, Zhu Hong-Liang, Zhang Hong-Xing, Xie Yuan-Hong, Jiang Wei-Bo, Zhao Xiao-Dan, Luo Kun-Bo

Abstract excerpt

Virus-induced gene silencing (VIGS) is an attractive reverse-genetics tool for studying gene function in plants. We showed that silencing of a phytoene desaturase (PDS) gene is maintained throughout TRV-PDS-inoculated tomato plants as well as in their flowers and fruit and is enhanced by low temperature (15 degrees C) and low humidity (30%). RT-PCR analysis of the PDS gene revealed a dramatic reduction in the...

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PMID: 16511359

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

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