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Article

A ligation-independent cloning tobacco rattle virus vector for high-throughput virus-induced gene silencing identifies roles for NbMADS4-1 and -2 in floral development.

Plant physiology - 1 Dec 2007

Dong Yiyu, Burch-Smith Tessa M, Liu Yule, Mamillapalli Padmavathi, Dinesh-Kumar Savithramma P

Abstract excerpt

Virus-induced gene silencing (VIGS) is a widely used, powerful technique for reverse genetics. VIGS vectors derived from the Tobacco rattle virus (TRV) are among the most popular for VIGS. We have developed a TRV RNA2 vector that allows the insertion of gene silencing fragments by ligation-independent cloning (LIC). This new vector has several advantages over previous vectors, particularly for applications...

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PMID: 17932306
DOI: 10.1104/pp.107.107391
PMCID: PMC2151726

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Source receipt: pubmed, retrieved 7 Sept 2026.

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