Article
Sprout vacuum-infiltration: a simple and efficient agroinoculation method for virus-induced gene silencing in diverse solanaceous species.
Plant cell reports - 1 Sept 2012
Yan Hua-xue, Fu Da-qi, Zhu Ben-zhong, Liu Hai-ping, Shen Xiao-ying, Luo Yun-bo
Abstract excerpt
UNLABELLED: Virus-induced gene silencing (VIGS) is a robust technique for identifying the functions of plant genes. Tobacco rattle virus (TRV)-mediated VIGS has been commonly used in many plants. In order to overcome the limitations of existing agroinoculation methods, we report an easy and effective method of agroinoculation for virus-induced gene silencing-sprout vacuum-infiltration (SVI). Using sprout...
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