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Article

Virus-induced gene silencing for in planta validation of gene function in cucurbits.

Plant physiology - 28 Nov 2022

Rhee Sun-Ju, Jang Yoon Jeong, Park Jun-Young, Ryu Jisu, Lee Gung Pyo

Abstract excerpt

Virus-induced gene silencing (VIGS) is a powerful tool for high-throughput analysis of gene function. Here, we developed the VIGS vector pCF93, from which expression of the cucumber fruit mottle mosaic virus genome is driven by the cauliflower mosaic virus 35S promoter to produce viral transcripts in inoculated plants. To test the utility of the pCF93 vector, we identified candidate genes related to male...

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PMID: 35944218
DOI: 10.1093/plphys/kiac363
PMCID: PMC9706489

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