Article
Virus-induced gene silencing as a tool for functional genomics in a legume species.
The Plant journal : for cell and molecular biology - 1 Nov 2004
Constantin Gabriela D, Krath Britta N, MacFarlane Stuart A, Nicolaisen Mogens, Johansen Ida Elisabeth, Lund Ole S
Abstract excerpt
Virus-induced gene silencing (VIGS) is an attractive reverse-genetics tool for studies of gene function. However, efficient VIGS has only been accomplished in a few plant species. In order to extend the application of VIGS, we examined whether a VIGS vector based on Pea early browning virus (PEBV) would produce recognizable phenotypes in Pisum sativum. A plasmid vector of PEBV was modified to allow...
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