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Article

CRISPR/Cas9-mediated Generation of Human Endothelial Cell Knockout Models of CCM Disease.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2020

Schwefel Konrad, Spiegler Stefanie, Much Christiane D, Felbor Ute, Rath Matthias

Abstract excerpt

The CRISPR/Cas9 system is a versatile tool that enables targeted genome editing in various cell types, including hard-to-transfect endothelial cells. The required crRNA, tracrRNA, and Cas9 protein have mostly been introduced into endothelial cells by viral transduction or plasmid transfection so far. We here describe an effective lipofection-based delivery of pre-complexed crRNA:tracrRNA:Cas9 ribonucleoproteins...

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Identifiers

PMID: 32524552
DOI: 10.1007/978-1-0716-0640-7_13

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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