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Article

Generating Mutant Renal Cell Lines Using CRISPR Technologies.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2020

Perretta-Tejedor Nuria, Freke Grace, Seda Marian, Long David A, Jenkins Dagan

Abstract excerpt

Gene editing using the CRISPR/Cas9 system is an extremely efficient approach for generating mutations within the genomic DNA of immortalized cell lines. This procedure begins with a straightforward cloning step to generate a single plasmid encoding the Cas9 enzyme as well as a synthetic guide RNA (sgRNA) which is selected to target specific sites within the genome. This plasmid is transfected into cells either...

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Identifiers

PMID: 31701460
DOI: 10.1007/978-1-4939-9841-8_20
PMCID: PMC9703199

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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