Article
High-throughput, microscope-based sorting to dissect cellular heterogeneity.
Molecular systems biology - 1 Jun 2020
Hasle Nicholas, Cooke Anthony, Srivatsan Sanjay, Huang Heather, Stephany Jason J, Krieger Zachary, Jackson Dana, Tang Weiliang, Pendyala Sriram, Monnat Raymond J, Trapnell Cole, Hatch Emily M, Fowler Douglas M
Abstract excerpt
Microscopy is a powerful tool for characterizing complex cellular phenotypes, but linking these phenotypes to genotype or RNA expression at scale remains challenging. Here, we present Visual Cell Sorting, a method that physically separates hundreds of thousands of live cells based on their visual phenotype. Automated imaging and phenotypic analysis directs selective illumination of Dendra2, a photoconvertible...
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