Article
Novel dot-blot assay for detection of vascular Notch3 aggregates in patients with CADASIL.
Journal of the neurological sciences - 15 Aug 2020
Ma Yihong, Ueda Mitsuharu, Ueda Akihiko, Shinriki Satoru, Nagatoshi Akihito, Isoguchi Aito, Okada Masamitsu, Tasaki Masayoshi, Nomura Toshiya, Inoue Yasuteru, Masuda Teruaki, Misumi Yohei, Yamashita Taro, Matsui Hirotaka, Ando Yukio
Abstract excerpt
To detect vascular Notch3 extracellular domain aggregates in CADASIL, we developed a novel dot-blot assay with both autopsy and biopsy skin samples. We obtained samples from 11 patients with CADASIL and 12 control patients, and we performed dot-blot analyses by using sequential biochemical tissue extractions with three different antibodies against specific regions of the Notch3 extracellular domain. We also...
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