Article
Bioethanol production from cellulosic hydrolysates by engineered industrial Saccharomyces cerevisiae.
Bioresource technology - 1 Mar 2017
Lee Ye-Gi, Jin Yong-Su, Cha Young-Lok, Seo Jin-Ho
Abstract excerpt
Even though industrial yeast strains exhibit numerous advantageous traits for the production of bioethanol, their genetic manipulation has been limited. This study demonstrates that an industrial polyploidy Saccharomyces cerevisiae JHS200 can be engineered through Cas9 (CRISPR associated protein 9)-based genome editing. Specifically, we generated auxotrophic mutants and introduced a xylose metabolic pathway into...
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