Article
An improved functional analysis of linker-mediated complex (iFALC) strategy.
Biochemical and biophysical research communications - 11 Jun 2020
Nakabayashi Yu, Harata Masahiko, Seki Masayuki
Abstract excerpt
The functional analysis of linker-mediated complex (FALC) strategy that facilitates functional analysis of a common subunit of multi-subunit protein complexes in cells constitutes three steps; (1) a common subunit is fused to a specific subunit via recombinant DNA, (2) mutation is introduced into a portion of the common subunit of the fused protein, and (3) the mutational effect on the fused protein is evaluated...
Topics
- Animals
- Cell Line
- Chickens
- Chromatin
- Gene Knockout Techniques
- Histones
- Multiprotein Complexes
- Mutant Proteins
- Mutation
- Protein Binding
- Protein Interaction Mapping