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Article

Highly efficient genome editing in N. gerenzanensis using an inducible CRISPR/Cas9-RecA system.

Biotechnology letters - 1 Sept 2020

Yue Xue, Xia Tianyu, Wang Shuai, Dong Huijun, Li Yongquan

Abstract excerpt

OBJECTIVE: To develop an inducible CRISPR/Cas9-Recombinase A (RecA) system to manipulate genes in Nonomuraea gerenzanensis effectively. RESULTS: Compared with traditional homologous recombination, the inducible CRISPR/Cas9 system achieved 68.8% editing efficiency, whereas, with both the inducible Cas9 and the overexpressed RecA, the efficiency of the combined genome editing system reached 100%. The dbv23-deleted...

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PMID: 32314149
DOI: 10.1007/s10529-020-02893-2

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