Article
Long-Read Nanopore Sequencing Validated for Human Leukocyte Antigen Class I Typing in Routine Diagnostics.
The Journal of molecular diagnostics : JMD - 1 Jul 2020
Matern Benedict M, Olieslagers Timo I, Groeneweg Mathijs, Duygu Burcu, Wieten Lotte, Tilanus Marcel G J, Voorter Christina E M
Abstract excerpt
Matching of human leukocyte antigen (HLA) gene polymorphisms by high-resolution DNA sequence analysis is the gold standard for determining compatibility between patient and donor for hematopoietic stem cell transplantation. Single-molecule sequencing (PacBio or MinION) is a newest (third) generation sequencing approach. MinION is a nanopore sequencing platform, which provides long targeted DNA sequences. The long...
Topics
- Alleles
- Base Sequence
- Data Accuracy
- Diagnostic Tests, Routine
- Genetic Testing
- HLA Antigens
- High-Throughput Nucleotide Sequencing
- Histocompatibility Antigens Class I
- Histocompatibility Testing
- Humans
- Nanopore Sequencing
- Nanopores