Using Nanopore Whole-Transcriptome Sequencing for Human Leukocyte Antigen Genotyping and Correlating Donor Human Leukocyte Antigen Expression with Flow Cytometric Crossmatch Results.

Abstract excerpt

Transplant centers are increasingly using virtual crossmatching to evaluate recipient and donor compatibility. However, the current state of virtual crossmatching fails to incorporate donor human leukocyte antigen (HLA) expression in the assessment, despite numerous studies that have demonstrated the impact of donor HLA expression on physical crossmatch outcomes. Whole-transcriptome sequencing (RNA-Seq) for HLA...

Topics

• Antibodies
• Blood Donors
• Cells, Cultured
• Flow Cytometry
• Genotype
• Genotyping Techniques
• HLA Antigens
• Histocompatibility Antigens Class I
• Histocompatibility Testing
• Humans