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Article

Otoferlin gene editing in sheep via CRISPR-assisted ssODN-mediated Homology Directed Repair.

Scientific reports - 7 Apr 2020

Menchaca A, Dos Santos-Neto P C, Souza-Neves M, Cuadro F, Mulet A P, Tesson L, Chenouard V, Guiffès A, Heslan J M, Gantier M, Anegón I, Crispo M

Abstract excerpt

Different mutations of the OTOF gene, encoding for otoferlin protein expressed in the cochlear inner hair cells, induces a form of deafness that is the major cause of nonsyndromic recessive auditory neuropathy spectrum disorder in humans. We report the generation of the first large animal model of OTOF mutations using the CRISPR system associated with different Cas9 components (mRNA or protein) assisted by single...

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Share to Clustered Regularly Interspaced Short Palindromic Repeats
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 32265471
DOI: 10.1038/s41598-020-62879-y
PMCID: PMC7138848

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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