Article
Structural and functional characterization of the dominant negative P-loop lysine mutation in the dynamin superfamily protein Vps1.
Protein science : a publication of the Protein Society - 1 Jun 2020
Tornabene Bryan A, Varlakhanova Natalia V, Hosford Christopher J, Chappie Joshua S, Ford Marijn G J
Abstract excerpt
Dynamin-superfamily proteins (DSPs) are large self-assembling mechanochemical GTPases that harness GTP hydrolysis to drive membrane remodeling events needed for many cellular processes. Mutation to alanine of a fully conserved lysine within the P-loop of the DSP GTPase domain results in abrogation of GTPase activity. This mutant has been widely used in the context of several DSPs as a dominant-negative to impair...
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