Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Unexpected NADPH Hydratase Activity in the Nitrile Reductase QueF from Escherichia coli.

Chembiochem : a European journal of chemical biology - 15 May 2020

Jung Jihye, Braun Jan, Czabany Tibor, Nidetzky Bernd

Abstract excerpt

The nitrile reductase QueF catalyzes NADPH-dependent reduction of the nitrile group of preQ0 (7-cyano-7-deazaguanine) into the primary amine of preQ1 (7-aminomethyl-7-deazaguanine), a biologically unique reaction important in bacterial nucleoside biosynthesis. Here we have discovered that the QueF from Escherichia coli-its D197A and E89L variants in particular (apparent kcat ≈10-2 min-1 )-also catalyze the slow...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Catalysis
Share to Cysteine
Share to Escherichia coli Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Hydro-Lyases

Share to Mutagenesis, Site-Directed

Share to Nitriles

Share to Oxidoreductases

Source

Open in PubMed Open full text

Identifiers

PMID: 31850614
DOI: 10.1002/cbic.201900679
PMCID: PMC7317782

Genes

C6
C5

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Unexpected NADPH Hydratase Activity in the Nitrile Reductase QueF from Escherichia coli.PMID 31850614Read ↗

Evidence of a sequestered imine intermediate during reduction of nitrile to amine by the nitrile reductase QueF from Escherichia coli.Semantic proximity Read ↗

Evidence that the metabolite repair enzyme NAD(P)HX epimerase has a moonlighting function.Semantic proximity Read ↗

Evolution of new enzymatic function by structural modulation of cysteine reactivity in Pseudomonas fluorescens isocyanide hydratase.Semantic proximity Read ↗

Structural and mechanistic views of enzymatic, heme-dependent nitrogen-nitrogen bond formationSemantic proximity Read ↗

Engineering the respiratory complex I to energy-converting NADPH:ubiquinone oxidoreductase.Semantic proximity Read ↗

A Suicide Mutation Affecting Proton Transfers to High-Valent Hemes Causes Inactivation of MauG during Catalysis.Semantic proximity Read ↗

A pathway for repair of NAD(P)H in plants.Semantic proximity Read ↗

Mutational study on alphaGln90 of Fe-type nitrile hydratase from Rhodococcus sp. N771.Semantic proximity Read ↗

Crystal structures of the ferrous dioxygen complex of wild-type cytochrome P450eryF and its mutants, A245S and A245T: investigation of the proton transfer system in P450eryF.Semantic proximity Read ↗

Enzyme-Substrate-Cofactor Dynamical Networks Revealed by High-Resolution Field Cycling Relaxometry.Semantic proximity Read ↗

Kinetic and structural studies on roles of the serine ligand and a strictly conserved tyrosine residue in nitrile hydratase.Semantic proximity Read ↗

Structure-function relationship of Vibrio harveyi NADPH-flavin oxidoreductase FRP: essential residues Lys167 and Arg15 for NADPH binding.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.