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Article

Probing the Impact of the Knob-into-Hole Mutations on the Structure and Function of a Therapeutic Antibody.

Analytical chemistry - 7 Jan 2020

Liu Peilu, Gao Xuan, Lundin Victor, Shi Catherine, Adem Yilma, Lin Kevin, Jiang Guoying, Kao Yung-Hsiang, Yang Feng, Michels David, Marshall Alan G, Zhang Hui-Min

Abstract excerpt

Bispecific antibodies (BsAbs) have drawn increasing interest in the biopharmaceutical industry due to their advantage to bind two distinct antigens simultaneously. The knob-into-hole approach is an effective way to produce bispecific antibodies by driving heterodimerization with mutations in the CH3 domain of each half antibody. To better understand the conformational impact by the knob and hole mutations, we...

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PMID: 31815436
DOI: 10.1021/acs.analchem.9b04855

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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