Article
Improvement in cyst recovery and molecular detection of Giardia duodenalis from stool samples.
Molecular biology reports - 1 Feb 2020
Bezagio Renata Coltro, Colli Cristiane Maria, Romera Liara Izabela Lopes, de Almeida Caroline Rodrigues, Ferreira Érika Cristina, Mattia Salete, Gomes Mônica Lúcia
Abstract excerpt
Molecular detection of Giardia duodenalis by polymerase chain reaction (PCR) is difficult in faecal samples due to inhibitors that contaminate DNA preparations, or due to low cyst concentrations. In order to eliminate inhibitors, improve cyst recovery and molecular detection of G. duodenalis, different types of water, distillates (MDs), deionized (MDz), injection (MI) or Milli-Q® (MM) were used instead of...
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