Article
Comparative analysis of routine parasitological methods for recovery of cysts, molecular detection, and genotyping of Giardia duodenalis.
European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology - 1 Dec 2021
Bezagio Renata Coltro, Colli Cristiane Maria, Romera Liara Izabela Lopes, de Almeida Caroline Rodrigues, Ferreira Érika Cristina, Gomes Mônica Lúcia
Abstract excerpt
In order to improve the diagnosis of giardiasis, fecal samples (high/medium/low concentration of cysts) were processed by the parasitological methods used in the routine: Faust, Lutz e Ritchie modified (replacement of formaldehyde by distilled water). The cysts were quantified; the DNA was extracted and amplified by semi-nested PCR (GDH gene). Fifteen clinical samples were analyzed to validate the study by...
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