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Comparison of efficiency of various DNA extraction methods from cysts of<i>Giardia intestinalis</i>measured by PCR and TaqMan real time PCR

1 Dec 2010

Abstract excerpt

The aim of the presented study was to work out an effective method of extraction of DNA from Giardia intestinalis cysts as well as a sensitive and specific method for detection of DNA of this protozoan using a polymerase chain reaction (PCR). Twelve protocols for DNA extraction have been compared. Purification and extraction of DNA were preceded by additional actions in order to destroy the cysts' wall. The...

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PMID: 21275235
DOI: 10.1051/parasite/2010174299

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