Article
Improved sensitivity of PCR amplification of glutamate dehydrogenase gene for detection and genotyping of Giardia duodenalis in stool specimen.
The Southeast Asian journal of tropical medicine and public health - 1 Mar 2010
Boontanom Parima, Siripattanapipong Suradej, Mungthin Mathirut, Tan-ariya Peerapan, Leelayoova Saovanee
Abstract excerpt
A modified set of primers was developed to increase the sensitivity of nested PCR amplification of glutamate dehydrogenase (gdh) gene to detect and genotype Giardia duodenalis cysts in stool specimens. This modified set of primers had a significantly higher sensitivity (82%) than that of a previously published PCR primer set (53%).
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