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Article

IMS-free DNA extraction for the PCR-based quantification of Cryptosporidium parvum and Giardia lamblia in surface and waste water.

International journal of environmental health research - 1 Aug 2007

Anceno Alfredo J, Katayama Hiroyuki, Houpt Eric R, Chavalitshewinkoon-Petmitr Porntip, Chuluun Buyan, Shipin Oleg V

Abstract excerpt

Extremely limited knowledge exists on the occurrence of protozoan pathogens in surface and waste water in the developing world. The article addresses one of the major reasons for this: prohibitively costly immunomagnetic separation (IMS) and commercial DNA extraction kits are required for the pathogen detection. As the presence of inhibitory substances critically impedes the polymerase chain reaction (PCR)-based...

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Source

Identifiers

PMID: 17613093
DOI: 10.1080/09603120701372573

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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