Article
Giardia intestinalis: DNA extraction approaches to improve PCR results.
Experimental parasitology - 1 Jun 2011
Babaei Zahra, Oormazdi Hormozd, Rezaie Sasan, Rezaeian Mostafa, Razmjou Elham
Abstract excerpt
Difficulty in disrupting cysts of Giardia intestinalis, a cosmopolitan protozoan parasite, decreases the yield of DNA extracted and reduces the effectiveness of the polymerase chain reaction (PCR). To improve the detection of the Giardia Glutamate Dehydrogenase (gdh) gene, we re-evaluated the effects of deoxyribonucleic acid (DNA) extraction methods. Purified and concentrated cysts from 33 fecal samples were...
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